Easytaq trans
WebThermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents. 10X Taq Buffer without Detergent (B55) includes: • 100 mM Tris-HCl (pH 8.8 at 25°C) • 500 mM KCl Related Products Taq Buffer (10X), with KCl WebEasyTaq ® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous …
Easytaq trans
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WebEasyTaq ® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq ® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. WebIn this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant’s tissue (1–10 μg) to 20 μl 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 °C for 15 min.
WebDriving Directions to Tulsa, OK including road conditions, live traffic updates, and reviews of local businesses along the way. Web2×EasyTaq® PCR SuperMix EasyTaq® PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq® DNA Polymerase for PAGE, dNTPs and optimized buffer. The SuperMix for PAGE is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. • Extension rate is about 1-2 kb/min. • Unique buffer system ...
WebEasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. Brand EasyTaq® Specificity The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. WebJun 15, 2024 · PCR amplification of each locus was performed in a 15 µL reaction system containing 1.5 µL 10 × TransStart ® TopTaq buffer (500 mM Tris-HCl, pH 9.0, 200 mM KCl, 200 mM (NH 4) 2 SO 4, and 20 mM MgSO 4 ), 1.2 µL dNTPs (2.5 mM), 0.3 µL each primer (10 µM), 0.3 µL TransStart ® TopTaq DNA Polymerase and about 10 ng total DNA.
WebEasyTaq®. Specificity. Extension rate is about 1-2 kb/min. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors. Amplification of genomic DNA fragment up to 4 kb. Characteristics. EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and ...
Web地址：北京市海淀区永泰庄北路1号中关村东升国际科学园4号楼. E-mail：[email protected]. 客服热线：+86-400 898 0321 cleaning my phonak hearing aidsWebView Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn Creek Township … cleaning my ninja air fryerWebDNA 聚合酶（EasyTaq、FastPfu 等）、电泳凝胶回收 试剂盒、酵母基因组抽提试剂盒等均购自 TransGen Biotech 公司。 主要仪器 电泳凝胶成像系统、BioRad 基因枪、PCR 仪、微量分光光度计等仪器 设备均由本实验室提供。 cleaning my own camera sensorWeb本产品包含EasyTaq ® DNA Polymerase、dNTPs和优化的反应缓冲液，浓度为2×。 DNA扩增时，只需加入模板、引物和水，使SuperMix溶液的浓度为1×即可进行反应。扩增产物3' 端带“A”碱基，2×EasyTaq ® PCR … cleaning my mindWeb产品详情介绍 EasyTaq® DNA Polymerase是从克隆有Thermu aquaticus DNA Polymerase基因的载体，在大肠杆菌中经诱导表达、分离纯化而成，其分子量为94 kDa … cleaning my oil diffuserWebTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. Brand TransStart® Characteristics - Extension rate is about 2-4 kb/min. - TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into pEASY®-Blunt vectors. cleaning my n64Web2*EasyTaq PCR SuperMix . EasyTaq ® SuperMix is a ready-to-use mixture of EasyTaq ® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. cleaning my phone storage