WebTo produce LacZ reporters under the control of the ABI5 promoter with a wild-type or mutant FBS motif, 39-bp oligonucleotides were synthesized as two complementary primers (ABI5wF and ABI5wR for the wild type and ABI5mF and ABI5mR for the mutant; for sequences, see Supplemental Table S1) with an EcoRI site overhang at the 5′ end and … Web(Lopez-Molina and Chua, 2000). rectly binds to the ABI5 promoter through the FBS motif, Although cross talk between ABA and light signaling both in vitro and in vivo, pathways has been observed (e.g. ABA metabolism in We next examined how FHY3 and FAR1 regulate
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WebFig. 2. FHY3 and FAR1 directly bind to the FBS motif present in the FHY1 and FHL promoters via the N-terminal zinc finger motif. (A) GAD-FHY3 and GAD-FAR1, but not GAD itself, strongly activate expression of the Loci reporter genes driven by the FHY1 and FHL promoters in yeast. (B) GAD-FHY3 and GAD-FAR1 activate the Lad reporter genes Web2 hours ago · Florida A&M EDGE Isaiah Land. Isaiah Land has generated some interest and buzz in the last two months as more scouts have taken a look at his tape. Land is very … WebYeast one-hybrid assay showed that GAD-FHY3 and GAD-FAR1 are capable of activating the LacZ reporter genes driven by PHYB, ELF4 and CCA1 promoter fragments containing the wild-type FBS motif, but ... conns ge appliances slate grey color