WitrynaThe hexahistidine-tagged organophosphorus hydrolase (OPH6His) has been immobilized on a Zr-MOF, namely UiO-66-NH2. The resulting enzyme-MOF composite was used as a carrier to facilitate the hydrolysis of an organophosphate pesticide, i.e., methyl parathion in to p-nitrophenol (PNP). The formation of PNP took place in direct … Witryna28 mar 2014 · Organophosphorus hydrolase (OPH) is a zinc-containing homodimeric protein identified in Flavobacterium sp. ATCC 27551 and Pseudomonas diminuta, isolated from soils in the Philippines and the United States, respectively, and has been named phosphotriesterase (PTE) [5, 24].
Extraction and Purification of Organophosphorus hydrolase …
WitrynaRecent global military events, such as the conflict in Syria, have emphasized the need to find effective strategies to rapidly destroy organophosphorus-based nerve agents. In … WitrynaMethods. Two animal models were used. First, Wistar rats were administered two different doses of the hydrolase (0.15 mg/kg and 1.5 mg/kg), and the ex vivo … famous hospital in chicago
Full article: Expression of recombinant organophosphorus hydrolase …
Witryna21 lut 2014 · Enhanced activity and stability of organophosphorus hydrolase via interaction with an amphiphilic polymer† Minkyu Kim , a Manos Gkikas , a Aaron … Major achievements in agriculture crop production have been obtained by using pesticides for successful pest control. One of the most popular types of pesticide is the organophosphate (OP) family, which effectively eliminates pests owing to its acute neurotoxicity. The effectiveness of OP compounds as … Zobacz więcej Since most of the important mutations responsible for improved hydrolysis occurred on or near the enzyme surface, molecular modeling was used to assess their potential … Zobacz więcej 1Department of Chemical and Environmental Engineering and 2Department of Biochemistry, University of California, Riverside, CA 92521, USA Zobacz więcej Witryna1 lis 2013 · Concerning that during the extraction procedure in presented study cells of lactobacilli remained in wheat samples, it is more likely that in studied lactic acid fermentation degradation of pirimiphos‐methyl occurred as a result of enzymatic activity, that is, biological degradation, most likely by gene encoding organophosphorus … famous hospitality inc